Anti-AP2A1 Polyclonal Antibody for WB, ELISA - O95782

Core Product Specifications and Parameters

Biological Background: AP2A1 Function and Localization

• AP2A1 encodes the alpha-1 subunit of the adaptor protein complex 2 (AP-2), also known as alpha-adaptin A, 100 kDa coated vesicle protein A, and clathrin assembly protein complex 2 alpha-A large chain.
• AP-2 functions in clathrin-dependent endocytosis, mediating cargo selection and vesicle formation at the plasma membrane, and recognizes Y-X-X-Φ and [ED]-X-X-X-L-[LI] endocytosis signals in transmembrane cargo tails.
• The AP-2 complex, via its alpha subunit, is responsible for ADAM10 endocytosis during long-term potentiation in hippocampal neurons (PubMed:23676497). Related references: PMID:23676497
• AP2A1 localizes to the cell membrane and clathrin-coated pits, consistent with its role in endocytic trafficking.
• Alternative splicing generates isoforms with different tissue distributions: isoform A is expressed in forebrain, skeletal muscle, spinal cord, and heart, while isoform B is widely expressed, including in breast and prostate cancer cells.
• AP2A1 is a phosphoprotein, suggesting regulation by post-translational modification.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-260) of human AP2A1; consider this when interpreting cross-reactivity with isoforms or species variants.
• For Western blot, use appropriate protein extraction methods and loading amounts given the ~108 kDa molecular weight; validate antibody specificity using positive and negative controls.
• For ELISA, optimize coating conditions and antibody dilutions in the relevant sample matrix.
• Since cross-reactivity is reported for human, mouse, and rat, the antibody may recognize conserved epitopes; verify performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, delivering validated antibodies for key targets such as AP2A1 in endocytosis and membrane trafficking. Beyond this polyclonal antibody, we offer matched antibody pairs, bulk ancillary reagents, and customized rare-target sourcing solutions. Our integrated support helps streamline your workflow from early-stage research to clinical application.