Anti-AP1M2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6Q5

Core Product Specifications and Parameters

Biological Background: AP1M2 Function and Localization

• AP1M2 encodes the mu-2 subunit of the clathrin-associated adaptor protein complex 1 (AP-1), which plays a critical role in protein sorting at the trans-Golgi network (TGN) and endosomes.
• The AP-1 complex mediates the recruitment of clathrin to membranes and recognizes sorting signals (such as tyrosine-based and dileucine motifs) within the cytosolic tails of transmembrane cargo molecules.
• AP1M2 is also known as mu1B-adaptin and belongs to the adaptor protein complex mu subunit family, which includes other medium chains like AP1M1 and AP2M1.
• The protein localizes to the Golgi apparatus and clathrin-coated vesicles, consistent with its function in vesicle-mediated transport between the TGN and endosomes.
• Post-translational modifications include phosphorylation, as indicated by the keyword “Phosphoprotein,” which may regulate cargo sorting activity.
• According to UniProt, keywords such as “Alternative splicing” and “Proteomics identification” suggest the existence of multiple isoforms and confirm endogenous expression.
• The calculated molecular weight of human AP1M2 is approximately 48 kDa, which aligns with the observed band in Western blot experiments.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 164–423 of human AP1M2, encompassing the mu homology domain. This region is highly conserved across species, supporting the reported cross-reactivity with mouse and rat.
• For Western blot (WB), expect a specific band around 48 kDa in lysates from human, mouse, or rat cells. Consider using HeLa or HEK293 as positive controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), validate staining in fixed and permeabilized cells. AP1M2 should exhibit a perinuclear Golgi-like pattern and punctate cytoplasmic vesicles.
• ELISA can be performed using recombinant AP1M2 protein or peptide antigens. Optimize coating concentrations and antibody dilutions for your specific assay conditions.

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CamelBio is a one-stop partner for IVD raw material sourcing, providing high-quality antibodies such as this anti-AP1M2 polyclonal antibody for research into intracellular trafficking and clathrin-mediated transport. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Let us streamline your supply chain from discovery to diagnostic development.