Anti-AP1M1 Polyclonal Antibody for WB and ELISA - Q9BXS5

Core Product Specifications and Parameters

Biological Background: AP1M1 Function and Localization

• The AP1M1 gene encodes the medium subunit mu-1 of the adaptor protein complex AP-1, also known as AP47, mu1A, or CLTNM.
• AP1M1 is a component of the heterotetrameric AP-1 complex, which plays a critical role in clathrin-mediated vesicle budding from the trans-Golgi network (TGN) and endosomes.
• The complex recognizes sorting signals, such as tyrosine-based motifs, on the cytosolic tails of transmembrane cargo proteins, directing their trafficking to specific compartments.
• AP1M1 is found in the Golgi apparatus and on clathrin-coated vesicle membranes, consistent with its role in intracellular transport.
• Post-translational modifications include phosphorylation and acetylation, which may regulate AP-1 complex assembly and function.
• Alternative splicing of the AP1M1 gene produces multiple isoforms, potentially contributing to functional diversity.
• The protein has been implicated in host-virus interactions, as indicated by its annotation in UniProt, although specific mechanisms remain to be fully elucidated.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-160) of human AP1M1, suggesting the antibody likely recognizes an epitope in this domain; performance should be validated in the target species and application.
• For western blotting, consider using whole-cell lysates or membrane fractions from human, mouse, or rat cell lines; the predicted molecular weight is 49kDa (human), though actual migration may vary due to post-translational modifications.
• In ELISA applications, optimize coating antigen and antibody concentration; the antibody may be used to capture or detect AP1M1 in complex samples if validated accordingly.
• Cross-reactivity with mouse and rat AP1M1 has been reported; however, sequence alignment of the immunogen region between species is recommended to assess potential epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to critical IVD raw materials, including verified antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. For protein trafficking and clathrin-mediated endocytosis research, we offer a range of AP-1 complex antibodies and related tools, backed by technical support from concept to clinic. Let us be your reliable partner in sourcing rare and validated raw materials for your diagnostic assay development.