Anti-AP1B1 Rabbit Polyclonal Antibody for WB, ELISA - Q10567

Core Product Specifications and Parameters

Biological Background: AP1B1 Function and Localization

• AP1B1 encodes the beta1 subunit of the clathrin-associated adaptor protein complex 1 (AP-1), a heterotetrameric complex also known as Golgi adaptor HA1/AP1 adaptin beta subunit. The complex is critical for protein sorting between the trans-Golgi network (TGN) and endosomes.
• AP-1 mediates clathrin recruitment to membranes and recognizes specific sorting signals within the cytosolic tails of transmembrane cargo proteins, facilitating their packaging into clathrin-coated vesicles (PubMed:31630791). Related references: PMID:31630791
• Other aliases include Beta-1-adaptin, ADTB1, and CLAPB2, reflecting its role as a beta-adaptin subunit of the adaptor complex.
• Subcellularly, AP1B1 localizes predominantly to the Golgi apparatus and to clathrin-coated vesicle membranes at the TGN and/or endosomes.
• The protein is widely expressed across tissues, consistent with its fundamental role in intracellular membrane trafficking.
• Post-translational modifications include acetylation and nitration, and alternative splicing generates multiple isoforms. The protein is implicated in disease; variants are associated with deafness and ichthyosis.
• The full-length human AP1B1 protein has a molecular weight of approximately 104.6 kDa, and it contains domains essential for cargo recognition and clathrin binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 700-800 of human AP1B1 (NP_001118.3), a region likely in the C-terminal domain involved in cargo or accessory protein binding. Consider using this antibody for Western blot detection of endogenous AP1B1 in human, mouse, and rat cell lysates; the expected band is around 105 kDa.
• For ELISA, titrate the antibody against the immunogen peptide or recombinant AP1B1 to determine optimal working dilution.
• As a polyclonal antibody, it may recognize multiple epitopes and could exhibit batch-to-batch variability; validate performance in the relevant sample system and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research labs, and institutes a streamlined single source for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and bespoke sourcing of rare targets. The AP1B1 polyclonal antibody supports studies in intracellular trafficking and clathrin-mediated sorting, complementing our comprehensive portfolio for membrane biology and disease research.