Anti-AOX1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q06278

Core Product Specifications and Parameters

Biological Background: AOX1 Function and Localization

• AOX1 (aldehyde oxidase 1) is a cytoplasmic molybdo-flavoenzyme belonging to the xanthine oxidase family, containing iron‑sulfur clusters (2Fe‑2S), FAD, and a molybdenum cofactor.
• It catalyzes the oxidation of a wide range of aldehydes and nitrogen‑containing heterocyclic compounds, playing a critical role in the metabolism of xenobiotics and drugs.
• AOX1 contributes to the bioactivation of prodrugs, such as famciclovir, by oxidizing 6‑deoxypenciclovir to the active antiviral penciclovir.
• The enzyme is involved in reactive oxygen species (ROS) homeostasis and may generate superoxide through one‑electron reduction of molecular oxygen.
• AOX1 may also participate in nitric oxide (NO) biosynthesis by reducing nitrite to NO using NADH or aldehydes as electron donors.
• Subcellular localization is primarily cytoplasmic.
• It is abundantly expressed in the liver, with lower levels in adipose tissue, lung, skeletal muscle, and pancreas.
• Post‑translational modifications identified by proteomics include phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment (amino acids 340–590) of human AOX1; while cross‑reactivity with mouse and rat has been observed, validation in the relevant sample system is recommended.
• For Western blot, the predicted molecular weight is approximately 148 kDa; consider using a low‑percentage acrylamide gel and extended transfer times for efficient detection.
• In immunofluorescence/ICC, optimize fixation and permeabilization protocols suitable for cytoplasmic proteins.
• ELISA applications may require titration of both coating antigen and antibody to determine optimal working concentrations.
• Liver tissue or hepatocyte‑derived cell lines are recommended as positive controls due to high endogenous AOX1 expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers and research laboratories, offering a comprehensive range of IVD raw materials including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For studies involving xenobiotic metabolism, drug metabolism, or oxidative stress research, our AOX1 antibody supports robust detection of aldehyde oxidase 1. From rare target sourcing to technical consulting, CamelBio streamlines your procurement from concept to clinic.