Anti-ANXA8L2 Rabbit pAb for WB and ELISA - Q5VT79

Core Product Specifications and Parameters

Biological Background: Annexin A8-like Protein 1 Function and Localization

• ANXA8L1 (also designated ANXA8L2) encodes annexin A8-like protein 1, a member of the annexin superfamily characterized by calcium-dependent phospholipid-binding properties.
• The protein contains multiple annexin repeat domains, which facilitate reversible association with negatively charged membrane phospholipids upon calcium binding.
• Alternative splicing of the ANXA8L1 gene generates multiple transcript variants, potentially producing isoforms with distinct regulatory or functional features.
• Metal-binding capabilities are indicated, pointing to interactions with divalent cations that may modulate protein conformation or membrane affinity.
• Annexins are broadly involved in membrane dynamics, including endocytosis, exocytosis, and cytoskeletal organization; ANXA8L1 likely contributes to these processes in specific cellular contexts.
• Proteomic identification (UniProt keyword “Proteomics identification”) supports its expression at the protein level in reference human tissues or cell lines.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against the full-length recombinant ANXA8L2 protein (aa 1–276), is suitable for western blotting (WB) and ELISA; performance should be validated in the end user’s specific sample system.
• For WB, consider using a denaturing gel and optimize blocking and antibody dilution conditions; include appropriate positive and negative controls (e.g., lysates from human, mouse, or rat cells/tissues expressing ANXA8L2).
• In ELISA applications, the antibody can serve as either capture or detection reagent; pairing with a monoclonal antibody against a non-overlapping epitope may enhance assay specificity.
• As with all polyclonal antibodies, lot-to-lot variability may occur; it is advisable to request retention samples or perform lot-specific qualification for critical IVD applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including this ANXA8L2 polyclonal antibody and related annexin-targeting reagents. We support diagnostic manufacturers, laboratories, and research institutes with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing—simplifying your transition from concept to clinic.