Anti-ANXA3 Polyclonal Antibody for WB - P12429

Core Product Specifications and Parameters

Biological Background: ANXA3 Function and Localization

• Annexin A3 (also known as 35-alpha calcimedin, lipocortin III, placental anticoagulant protein III) is a member of the annexin family of calcium/phospholipid-binding proteins.
• It acts as an inhibitor of phospholipase A2, reducing the release of arachidonic acid from membrane phospholipids and thereby modulating inflammatory responses.
• ANXA3 possesses anti-coagulant properties, functioning as a placental anticoagulant protein that may play a role in maintaining placental hemostasis.
• The protein also demonstrates inositol 1,2-cyclic phosphate 2-phosphohydrolase activity, cleaving the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
• Key post-translational modifications include acetylation and phosphorylation, as indicated by proteomics and direct protein sequencing data.
• The annexin A3 structure contains characteristic annexin repeat domains, and the protein has been resolved in 3D structural studies.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human ANXA3 protein (aa 1-323), which may enable the polyclonal antibody to recognize multiple epitopes in denaturing Western blot conditions.
• For Western blot, expect a band at approximately 36 kDa; include appropriate positive and negative controls to confirm specificity.
• In ELISA, this antibody may be used as a capture or detection reagent; however, optimal dilutions and conditions should be experimentally determined.
• Reported cross-reactivity with mouse and rat should be validated in the relevant sample system before quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your premier one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-ANXA3 antibody, and bulk ancillary reagents. From coagulation studies to phospholipid signaling research, we supply high-quality raw materials to support your diagnostic development. With expertise in rare target sourcing, we bridge the gap from concept to clinic.