Anti-ANXA3 Polyclonal Antibody for WB and ELISA - P12429

Core Product Specifications and Parameters

Biological Background: Annexin A3 Function and Localization

• Annexin A3 (ANXA3), also known as annexin III, lipocortin III, or placental anticoagulant protein III (PAP-III), is a member of the annexin family of calcium-dependent phospholipid-binding proteins.
• It functions as an inhibitor of phospholipase A2, an enzyme that releases arachidonic acid from membrane phospholipids, thus modulating inflammatory responses.
• ANXA3 exhibits anti-coagulant properties, as reflected by its alias placental anticoagulant protein III, potentially by competing for phospholipid surfaces required in the coagulation cascade.
• The protein also acts as an inositol 1,2-cyclic phosphate 2-phosphohydrolase, cleaving the cyclic phosphate bond to generate inositol 1-phosphate.
• Structurally, annexin A3 contains characteristic annexin repeat domains that mediate calcium and phospholipid binding, and is subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the entire length of human ANXA3 (amino acids 1–323), which may yield reactivity against multiple epitopes; consider this when interpreting banding patterns in Western blot.
• For WB, the predicted molecular weight is approximately 36 kDa; use an appropriate gel percentage and transfer protocol to resolve this low-molecular-weight protein.
• Validate antibody specificity using positive and negative control lysates from human and rat tissues or cell lines known to express ANXA3.
• For ELISA applications, explore pairing with a matched detection antibody if developing a sandwich assay; perform titration experiments to optimize coating and detection concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with premium reagents like this ANXA3 polyclonal antibody for coagulation and inflammation research. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, alongside flexible sourcing services for rare targets to accelerate your assay development pipeline.