Anti-ANXA13 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P27216

Core Product Specifications and Parameters

Biological Background: ANXA13 Function and Localization

• ANXA13 encodes annexin A13, a member of the annexin family of calcium-dependent phospholipid-binding proteins. Two isoforms are produced by alternative splicing: isoform A (canonical) and a variant with distinct calcium sensitivity.
• Isoform A binds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner, with half-maximal binding at ~60 µM Ca²⁺. It does not associate with membranes lacking acidic phospholipids. Related references: PMID:27676605 PMID:30610115
• The alternative isoform also requires calcium for membrane interaction but exhibits lower affinity, requiring ~320 µM Ca²⁺ for half-maximal binding.
• Subcellularly, ANXA13 localizes to the apical cell membrane, cell membrane, and cytoplasmic vesicles, consistent with a role in membrane trafficking and organization.
• ANXA13 is predominantly expressed in intestinal epithelial cells, with protein detected in colon and jejunum, supporting its alias "Intestine-specific annexin" (ISA).
• The protein undergoes myristoylation (a lipid modification) and belongs to the annexin repeat family, characterized by conserved calcium- and phospholipid-binding domains.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1-316 of human ANXA13 (full-length canonical sequence). This may enable recognition of both isoforms, but verification is advised.
• For western blot (WB), the predicted molecular weight is ~35 kDa. Use whole-cell lysates or membrane fractions from intestinal cell lines (e.g., Caco-2, HT-29) or tissue homogenates (colon, jejunum).
• For immunohistochemistry (IHC-P), antigen retrieval protocols and antibody dilution should be optimized in positive control tissues such as human colon or jejunum sections.
• In ELISA applications, the antibody can be used for direct or indirect detection of recombinant or native ANXA13, with appropriate secondary antibodies and colorimetric/fluorescent substrates.
• Cross-reactivity with mouse and rat ANXA13 is indicated; however, users should validate in the relevant sample system to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a streamlined supply of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying annexin‑mediated membrane dynamics or intestinal epithelial biology, our team can source rare‑target antibodies like ANXA13 and provide technical consultation to accelerate assay development. Whether you need a single high‑quality antibody or a complete raw‑material solution, CamelBio is your collaborative partner from concept to clinic.