Anti-ANXA1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04083

Core Product Specifications and Parameters

Biological Background: ANXA1 Function and Localization

• ANXA1, also known as Annexin I, Lipocortin I, or Calpactin II, is a member of the annexin superfamily of calcium- and phospholipid-binding proteins.
• It functions as a key regulator of the innate immune response, exhibiting anti-inflammatory activity by mediating glucocorticoid effects and down-regulating early-phase inflammation. Related references:
  • PMID:8425544
• ANXA1 contributes to adaptive immunity by modulating T-cell activation: it enhances signaling cascades upon T-cell activation, promotes differentiation into Th1 cells, and negatively regulates Th2 differentiation. Related references:
  • PMID:17008549
• It binds calcium with high affinity (up to eight Ca2+ ions) and associates with phospholipid membranes in a Ca2+-dependent manner, participating in phagocytic cup formation and actin cytoskeleton reorganization during phagocytosis. Related references:
  • PMID:2532504
  • PMID:8557678
• ANXA1 activates formyl peptide receptors (FPRs) to promote chemotaxis of granulocytes and monocytes, cell polarization, migration, and the resolution of inflammation and wound healing. Related references:
  • PMID:15187149
  • PMID:22879591
  • PMID:25664854
• In the context of antitumor immunity, ANXA1 interacts with FPR1 on dendritic cells to facilitate tumor-associated antigen uptake and cross-presentation, thereby mounting specific T-cell responses.
• Subcellularly, it localizes to the nucleus, cytoplasm, cell membrane, endosomes, phagocytic cups, secretory vesicles, and is also secreted into the extracellular space and exosomes.
• Tissue expression includes resting neutrophils, peripheral blood T cells, placenta, and liver, with detection in extracellular vesicles from inflammatory bowel disease patient serum.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human ANXA1 protein (residues 1-346); the polyclonal nature provides robust epitope coverage, but cross-reaction with other annexin family members should be considered if sequence similarity exists.
• For Western blot (WB), the predicted band is ~39 kDa; note that post-translational modifications (e.g., phosphorylation, acetylation) may cause slight shifts. Validate in the relevant sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions, as ANXA1 can exist in membrane-associated and cytosolic pools. A suitable fixation method should preserve this distribution.
• ELISA applications may require antibody titration and blocking against non-specific binding; pair with relevant controls.
• Cross-reactivity with rat ANXA1 is reported, but sequence alignment is advised for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and research institutes from concept to clinical application. This high-quality anti-ANXA1 polyclonal antibody is suitable for studies on inflammation, innate immunity, and glucocorticoid-mediated pathways. As part of our integrated supply solutions, we offer validated antibody pairs, recombinant proteins, bulk ancillary reagents, and custom rare-target sourcing to streamline your assay development and production.