Anti-ANT1/ANT2/ANT3/ANT4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P12235 / P05141 / P12236 / Q9H0C2

Core Product Specifications and Parameters

Biological Background: ANT1/ANT2/ANT3/ANT4 Function and Localization

• ANT1 (SLC25A4) is an ADP/ATP antiporter that catalyzes the exchange of mitochondrial matrix ATP for cytosolic ADP, a central step in cellular energy metabolism.
  Related references: PMID:21586654 PMID:27693233 PMID:23173940
• The protein operates via an alternating access mechanism, cycling between cytoplasmic-open and matrix-open states to alternately expose a single substrate-binding site to either side of the inner mitochondrial membrane.
• ANT1 also mediates mitochondrial uncoupling by acting as a proton transporter, a process that diminishes ATP synthesis efficiency and contributes to thermogenesis; this activity requires free fatty acids as cofactors.
• It participates in the mitochondrial permeability transition pore (mPTP), a non-specific pore implicated in cell death.
  Related references: PMID:31883789
• Independently of its transport function, ANT1 regulates mitophagy by interacting with TIMM44, which inhibits the TIM23 translocase and stabilizes PINK1.
• The protein resides in the mitochondrial inner membrane and is subject to multiple post-translational modifications, including acetylation, methylation, phosphorylation, and S-nitrosylation.
• The antibody recognizes all four adenine nucleotide translocase isoforms (ANT1–4), which share conserved sequences in the C-terminal region.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a synthetic peptide from the C-terminus of human ANT1, a region highly conserved among ANT isoforms, which likely accounts for its broad reactivity with ANT1–4.
• For Western blot, expected bands are around 33 kDa (ANT1), 32 kDa (ANT2/3), and 35 kDa (ANT4); use appropriate loading controls and validate band identity with knockdown or knockout samples.
• In IHC-P, optimize antigen retrieval and antibody dilution on known positive control tissues (e.g., heart or skeletal muscle) before analyzing experimental samples.
• For ELISA, consider using the peptide immunogen as a positive coating antigen to verify antibody titer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a comprehensive range of IVD raw materials, including validated monoclonal and polyclonal antibodies, antigen-grade proteins, and ancillary reagents. This anti-ANT1/2/3/4 polyclonal antibody supports mitochondrial metabolism and cell death research, and we can assist with bulk supply, custom conjugation, or immunogen design to accelerate your assay development pipeline.