Anti-ANPEP/CD13 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P97449

Core Product Specifications and Parameters

Biological Background: Aminopeptidase N (CD13) Function and Localization

• Aminopeptidase N (APN/CD13) is a broad-specificity membrane-bound zinc metalloprotease that participates in the final digestion of peptides and the processing of peptide hormones, neuropeptides, and chemokines (by similarity).
• It cleaves peptides bound to major histocompatibility complex class II molecules on antigen‑presenting cells, suggesting a role in antigen presentation. Related references: PMID:8691132
• APN may also function in angiogenesis and promote cholesterol crystallization (by similarity).
• It acts as a binding partner of the amino acid transporter SLC6A19 and regulates its activity, contributing to amino acid transport. Related references: PMID:22677001
• The protein is localized to the cell membrane and is highly expressed in the intestinal brush border, kidney, liver, and on immune cells including monocytes, macrophages, dendritic cells, and B cells (but not T cells). Related references: PMID:22677001, PMID:8103749, PMID:8805662
• Post‑translational modifications include N‑glycosylation, phosphorylation, disulfide bond formation, and tyrosine sulfation.
• It contains a zinc‑binding motif typical of the M1 family of metalloproteases and is anchored to the membrane via an N‑terminal signal‑anchor sequence.

Experimental Guidance and Technical Tips

• The immunogen (aa 69–966) covers the majority of the extracellular domain; ensure your detection system recognizes this region if using epitope‑specific reagents.
• For Western blot, a band at approximately 109 kDa is expected; use appropriate molecular weight markers and loading controls.
• In immunofluorescence (IF‑P) and immunohistochemistry (IHC‑P), fixation and permeabilization protocols should be optimized for membrane proteins; antigen retrieval may be necessary for paraffin‑embedded tissues.
• Cross‑reactivity with human and rat CD13 is reported; validate in the relevant species and tissue before quantitative analysis.
• For ELISA, consider pairing with a detection antibody recognizing a non‑overlapping epitope if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This ANPEP/CD13 antibody is suited for studies in immunology, nephrology, and oncology research. Whether you need rare‑target antibodies, custom recombinant proteins, or technical consulting, CamelBio serves as your strategic sourcing partner from concept to clinic.