Anti-ANP32E Rabbit Polyclonal Antibody for WB, ELISA - Q9BTT0

Core Product Specifications and Parameters

Biological Background: ANP32E Function and Localization

• ANP32E (Acidic leucine-rich nuclear phosphoprotein 32 family member E), also known as LANP-like protein (LANP-L), belongs to the ANP32 family of histone chaperones.
• It functions as a specific histone chaperone that mediates the genome-wide removal of histone variant H2A.Z from nucleosomes, particularly at enhancer and insulator regions, and stabilizes the evicted H2A.Z-H2B dimer (PubMed:24463511). Related references: PMID:24463511
• ANP32E inhibits the activity of protein phosphatase 2A (PP2A) and may play a role in cerebellar development and synaptogenesis.
• The protein localizes to both the cytoplasm and nucleus.
• It is expressed in a broad range of tissues, including peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver, and kidney.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, and the protein contains leucine-rich repeats.
• Alternative splicing produces multiple isoforms of ANP32E.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates from human, mouse, or rat tissues/cell lines known to express ANP32E (e.g., leukocytes, colon, skeletal muscle). A band at approximately 31 kDa is expected; however, due to alternative splicing, multiple bands may be observed. Validate antibody specificity with appropriate controls.
• In ELISA, use recombinant ANP32E protein or peptide coating and optimize antibody dilution empirically. Cross-reactivity with mouse and rat ANP32E may be assessed using orthologous antigens.
• The immunogen comprises the N-terminal 70 amino acids of human ANP32E. Epitope accessibility may be influenced by post-translational modifications or protein interactions; consider denaturing conditions for WB.
• Always include positive and negative controls, and validate in the relevant sample system to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with streamlined access to a broad portfolio of raw materials, including antibodies against epigenetic targets such as the histone chaperone ANP32E. We offer validated ANP32E polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to support diagnostic assay development. For rare or custom targets in chromatin biology, our sourcing and technical consultation services help ensure a reliable supply chain from concept to clinic.