Anti-ANP32B Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - Q92688

Core Product Specifications and Parameters

Biological Background: ANP32B Function and Localization

• ANP32B (acidic leucine-rich nuclear phosphoprotein 32 family member B) is also known as APRIL, SSP29, and PHAPI2. It belongs to the ANP32 family of proteins characterized by leucine-rich repeats.
• It functions as a multifunctional regulator of cell proliferation, apoptosis, cell cycle progression, and transcription. For example, it regulates neuronal stem cell proliferation and leukemic cell differentiation, and acts as a negative regulator of caspase-3-dependent apoptosis, possibly antagonizing ANP32A. Related references: PMID:18039846 PMID:20015864
• ANP32B exhibits histone chaperone activity, recruiting histones to specific gene promoters and thereby modulating transcription. Related references: PMID:18039846 PMID:20538007
• It plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the XPO1/CRM1 export receptor. Related references: PMID:17178712
• (Microbial infection) ANP32B is crucial for influenza A and B viral genome replication and contributes to foamy virus mRNA export. Related references: PMID:31217244 PMID:33045004 PMID:21159877
• Subcellular localization includes the nucleus, cytoplasm, and possibly other cellular compartments.
• It is expressed in heart, lung, pancreas, prostate, spleen, thymus, and placenta.
• Post-translational modifications include acetylation and phosphorylation. The protein contains leucine-rich repeats and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human ANP32B. When performing Western blotting, consider using lysates from human, mouse, or rat cell lines and expect a specific band near 29 kDa.
• For immunofluorescence applications (IF/ICC, IF-P), the protein localizes to both nucleus and cytoplasm, so optimize fixation and permeabilization conditions accordingly.
• Due to high sequence conservation across human, mouse, and rat, the antibody may cross-react with these species, as indicated; validate in your sample system if needed.
• For ELISA, the antibody can be used as a detection or capture reagent; pairing with a suitable matched antibody is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials, providing comprehensive support from concept to clinic. This anti-ANP32B rabbit monoclonal antibody exemplifies our offerings for research in apoptosis, cell cycle regulation, and viral replication. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance with rare target raw-material procurement, our team ensures reliable supply to accelerate your diagnostic development.