Anti-ANO2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NQ90

Core Product Specifications and Parameters

Biological Background: ANO2 Function and Localization

• ANO2 (Anoctamin-2), also known as Transmembrane protein 16B (TMEM16B), is a member of the anoctamin family of calcium-activated chloride channels.
• It functions as a calcium-activated chloride channel (CaCC) that mediates chloride ion transport upon increases in intracellular calcium levels.
• In olfactory sensory neurons, ANO2 amplifies the odorant-induced depolarization by providing the CaCC current essential for olfactory signal transduction.
• In the retina, ANO2 is expressed in photoreceptor synaptic terminals and may contribute to the amplification of light perception.
• The protein is localized to the cell membrane and contains multiple transmembrane helices typical of ion channels.
• Post-translational modifications include glycosylation (as indicated by the keyword Glycoprotein) and the presence of multiple isoforms generated by alternative splicing.
• ANO2 is predominantly expressed in the retina, particularly in photoreceptor synaptic terminals, highlighting its role in sensory transduction.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1-200) of human ANO2, which may recognize multiple isoforms.
• For Western blotting, a band around 114 kDa is expected; consider using reduced denaturing conditions and appropriate molecular weight markers.
• In immunocytochemistry/immunofluorescence, ANO2 shows cell membrane localization; include appropriate controls to verify specificity.
• This antibody has been reported to react with human, mouse, and rat samples; validate the antibody in your specific experimental system.
• For ELISA, titrate the antibody to obtain an optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to premium IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for assay development. This anti-ANO2 polyclonal antibody is ideal for studies on calcium-activated chloride channels in olfactory and visual signal transduction. For bulk orders, custom formulations, or technical consultation, our team offers end-to-end support from concept to clinic.