Anti-Annexin A6/Annexin VI Polyclonal Antibody for WB, ELISA - P08133

Core Product Specifications and Parameters

Biological Background: Annexin A6 Function and Localization

• Annexin A6, also designated annexin VI, calphobindin-II, chromobindin-20, lipocortin VI, p68, or p70, is encoded by the ANXA6 gene and belongs to the conserved annexin family of calcium-dependent phospholipid-binding proteins.
• It localizes to the cytoplasm and melanosomes, and it may associate with CD21, implicating it in membrane-related processes and intracellular signaling.
• Alternative splicing generates multiple isoforms, reflected by distinct molecular weights (e.g., 67 kDa, 76 kDa) and alternative names.
• Post-translational modifications such as acetylation and phosphorylation are observed, which could modulate its activity.
• As an annexin, it possesses characteristic repeat domains that mediate calcium and phospholipid binding, crucial for membrane interaction.
• It may regulate Ca²⁺ release from intracellular stores, indicating involvement in calcium homeostasis.
• Its presence in melanosomes suggests a potential role in pigmentation or organelle trafficking.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–250 of human annexin A6; thus, the antibody likely recognizes epitopes within this N-terminal region.
• In Western blotting, a band at approximately 76 kDa is expected under standard reducing and denaturing conditions.
• Because the immunogen spans a conserved domain, cross-reactivity with other annexin family members should be ruled out using knockout or knockdown controls.
• For ELISA applications, titrate the antibody and optimize coating conditions to suit the specific assay format.
• This antibody reacts with human and mouse samples; for other species, consult sequence alignment of the immunogen region to predict recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories seamless access to key IVD raw materials, from validated antibodies to bulk ancillary reagents. For Annexin A6-related research, we provide high-quality monoclonal and polyclonal antibodies, optimized antibody pairs, and large-scale sourcing to support your assay development needs. Let us help bridge your project from concept to clinic with a tailored procurement strategy.