Anti-Annexin A6/Annexin VI Monoclonal Antibody for WB, IHC-P, ELISA - P08133

Core Product Specifications and Parameters

Biological Background: Annexin A6/Annexin VI Function and Localization

• Annexin A6 (also known as Annexin VI, p68, p70, calphobindin-II) is a member of the annexin family of calcium-dependent phospholipid-binding proteins. The gene ANXA6 encodes multiple isoforms through alternative splicing.
• It is thought to associate with the complement receptor CD21 and may play a role in regulating the release of Ca²⁺ from intracellular stores.
• Subcellularly, Annexin A6 is predominantly localized to the cytoplasm and has been identified in melanosomes.
• The protein contains characteristic annexin repeats, enabling reversible binding to membrane phospholipids in a Ca²⁺-regulated manner.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity and interactions.
• Key functional contexts include membrane trafficking, signal transduction, and calcium homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 324–673) of human Annexin A6, which should recognize full-length protein and possibly conserved epitopes across species given reported cross-reactivity with mouse and rat.
• For Western blot (WB), the predicted molecular weight is ~76 kDa. Use reducing denaturing conditions; validate detection with appropriate positive controls and loading controls.
• In immunohistochemistry (paraffin-embedded sections, IHC-P), antigen retrieval may improve epitope exposure. Test a range of conditions to optimize signal.
• For ELISA applications, the antibody may be used as capture or detection reagent; pairing with another clone or polyclonal antibody could enhance sensitivity in sandwich assays.
• Because Annexin A6 has calcium-dependent membrane binding, consider including calcium in assay buffers where relevant to preserve native interactions, though denaturing assays (WB) may not require it.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop procurement of IVD raw materials, from optimized monoclonal antibodies like this anti-Annexin A6 clone to bulk ancillary reagents. Building on reliable supply chains, we help you accelerate development of assays targeting calcium signaling and membrane trafficking pathways. For projects requiring rare targets or custom immunogens, our sourcing network and technical consulting bridge the gap from concept to clinical application.