Anti-Annexin A5/Annexin V Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08758

Core Product Specifications and Parameters

Biological Background: Annexin A5 Function and Localization

• Annexin A5, also known as Annexin V, is a member of the annexin family of calcium-dependent phospholipid-binding proteins.
• It functions as an anticoagulant by indirectly inhibiting the thromboplastin-specific complex in the blood coagulation cascade.
• The protein is known by multiple alternative names including Anchorin CII, Calphobindin I (CPB-I), Endonexin II, Lipocortin V, and PP4.
• According to UniProt keywords, it undergoes various post-translational modifications such as acetylation, phosphorylation, S-nitrosylation, ubiquitination, and isopeptide bond formation.
• It contains internal repeats characteristic of the annexin family and requires calcium ions for phospholipid binding.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-320 of human Annexin A5, which represents the full-length protein; this polyclonal antibody may recognize multiple epitopes.
• For Western blot (WB), the predicted molecular weight is approximately 36 kDa; consider optimizing the antibody dilution and running appropriate controls to verify specificity.
• This antibody has been validated by knockout (KO) studies, providing confidence in target specificity; however, independent validation in your specific experimental system is recommended.
• Cross-reactivity with human and mouse is reported; for other species, sequence homology suggests possible reactivity but should be tested empirically.
• In immunofluorescence (IF/ICC) and ELISA applications, standard protocols may be followed; ensure appropriate fixation and permeabilization methods for IF to preserve epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focused on coagulation and hemostasis, this Annexin A5/Annexin V antibody serves as a reliable tool for probing thromboplastin complex regulation. With a comprehensive portfolio and rare-target sourcing capabilities, CamelBio supports every stage from concept to clinic.