Anti-Annexin A5/Annexin V Rabbit Monoclonal Antibody for WB, ELISA - P08758

Core Product Specifications and Parameters

Biological Background: Annexin A5 Function and Localization

• Annexin A5, also known as Annexin V, Lipocortin V, Endonexin II, Calphobindin I, placental anticoagulant protein 4 (PP4), thromboplastin inhibitor, and vascular anticoagulant-alpha (VAC-alpha), is a 35.9 kDa member of the annexin family of calcium-dependent phospholipid-binding proteins.
• It functions as an anticoagulant by acting as an indirect inhibitor of the thromboplastin-specific complex in the blood coagulation cascade.
• The protein belongs to the annexin superfamily, characterized by conserved annexin repeats that mediate calcium and phospholipid binding.
• Post-translational modifications include acetylation, phosphorylation, S-nitrosylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its activity.
• Keywords associated with Annexin A5 include hemostasis, blood coagulation, and calcium/phospholipid-binding, underscoring its role in maintaining vascular homeostasis.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide spanning amino acids 221-320 (C-terminal region), is expected to generate antibodies recognizing the native protein in multiple species, consistent with the reported human, mouse, and rat cross-reactivity.
• For Western blot (WB), anticipate a band at approximately 36 kDa; consider optimizing sample preparation and loading to account for potential post-translational modifications that may cause slight shifts.
• For ELISA, the antibody may be suitable for both direct and sandwich formats; pilot experiments with appropriate antigen standards are recommended to establish sensitivity and specificity.
• Although not listed in the specifications, immunofluorescence or immunohistochemistry may be attempted with additional validation, as Annexin A5 has been localized to cell surfaces and intracellular compartments under certain conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement by offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert technical support. This Annexin A5 antibody supports hemostasis, thrombosis, and cardiovascular research, enabling diagnostic manufacturers and laboratories to accelerate assay development from discovery to clinical application. As your one-stop sourcing bridge, CamelBio ensures reliable supply and tailored solutions for even rare-target raw materials.