Anti-Annexin A5/Annexin V Polyclonal Antibody for WB, IHC-P, ELISA - P08758

Core Product Specifications and Parameters

Biological Background: Annexin A5 Function and Localization

• Annexin A5 (also known as Annexin V, Anchorin CII, Calphobindin I, Endonexin II, Lipocortin V, etc.) is a member of the annexin family of calcium-dependent phospholipid-binding proteins.
• It functions as an anticoagulant protein by acting as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
• The protein plays a key role in hemostasis and the regulation of blood clotting.
• It binds to phosphatidylserine in a calcium-dependent manner, enabling its use as a marker for apoptotic cells in vitro and in vivo.
• Post-translational modifications include acetylation, phosphorylation, S-nitrosylation, and ubiquitin conjugation (isopeptide bond).
• The molecular weight of the mature protein is approximately 36 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to the full-length human Annexin A5 (aa 1-320). The polyclonal antibody is expected to recognize multiple epitopes, providing robust detection in various applications.
• For Western blot (WB), consider optimizing antibody dilution and using appropriate blocking conditions to minimize background. The predicted band size is ~36 kDa, but may appear slightly different depending on sample preparation.
• For immunohistochemistry (IHC-P), antigen retrieval methods may be required. Validate staining specificity with appropriate controls.
• ELISA sensitivity may vary; titrate the antibody to determine optimal working concentration for your assay format.
• The antibody cross-reacts with mouse and rat, making it suitable for studies in these model organisms. However, verify cross-reactivity in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner for diagnostic manufacturers, labs, and research institutes. This Annexin A5 polyclonal antibody is ideal for blood coagulation and apoptosis research applications, and CamelBio can assist with bulk orders, validated antibody pairs, and rare-target reagent sourcing. Contact us to learn more about our comprehensive portfolio and custom supply solutions.