Anti-Annexin A4/Annexin IV Rabbit Polyclonal Antibody for WB and ELISA - P09525

Core Product Specifications and Parameters

Biological Background: Annexin A4/Annexin IV Function and Localization

• Annexin A4 (also known as Annexin IV, 35-beta calcimedin, PAP-II, among others) is a member of the annexin family of calcium-dependent phospholipid-binding proteins.
• It functions primarily as a calcium/phospholipid-binding protein that promotes membrane fusion and is critically involved in exocytosis.
• The protein localizes to the membrane of zymogen granules, consistent with its role in regulated secretion and membrane trafficking.
• Post-translational modifications, including acetylation and phosphorylation, are reported and may modulate its membrane-binding activity or protein interactions.
• Multiple isoforms can arise from alternative initiation and alternative splicing, potentially expanding functional diversity.
• Structurally, Annexin A4 contains characteristic annexin repeats that facilitate its calcium-dependent interaction with negatively charged phospholipids on cellular membranes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 112–321 of human Annexin A4, a region downstream of the core annexin domains; this design may favor detection of the full-length protein.
• For Western blot, a primary band is anticipated at approximately 36 kDa; additional bands from modified or alternative isoforms may be observed depending on sample type.
• In ELISA, consider using recombinant Annexin A4 as a standard and titrate the antibody against target-coated wells to establish a working dilution.
• Due to documented cross-reactivity with mouse, this antibody can likely be applied to mouse-derived samples, but independent validation in the relevant tissue or cell line is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material procurement, offering comprehensive support from concept to clinic. For Annexin A4 research and diagnostics—spanning exocytosis, membrane biology, and calcium signaling—we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our global sourcing network also enables efficient access to rare-target raw materials and custom development, ensuring streamlined supply chains for diagnostic manufacturers and laboratories.