Anti-Annexin A4/Annexin IV Rabbit Monoclonal Antibody for WB, ELISA - P09525

Core Product Specifications and Parameters

Biological Background: Annexin A4/Annexin IV Function and Localization

• Annexin A4 (Annexin IV) is a member of the annexin family of calcium-dependent phospholipid-binding proteins, also known as 35-beta calcimedin, endonexin I, lipocortin IV, and several other aliases.
• It functions in promoting membrane fusion and participates in exocytosis, binding to phospholipids in a calcium-dependent manner.
• The protein contains characteristic annexin repeats that mediate its interaction with acidic phospholipids.
• Subcellularly, Annexin A4 localizes to the zymogen granule membrane, highlighting its role in regulated secretion.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity.
• Alternative initiation and alternative splicing generate multiple isoforms, expanding functional diversity.
• The protein has been studied by X-ray crystallography (3D structure available) and identified in various proteomics studies.

Experimental Guidance and Technical Tips

• For Western Blot, anticipate a band at approximately 36 kDa. Validate detection using positive controls such as human, mouse, or rat cell lysates known to express Annexin A4.
• As a monoclonal antibody, it may be suitable for quantitative ELISA; however, users should confirm linearity and specificity in their own assay format.
• Cross-reactivity with mouse and rat enables comparative studies; always include species-appropriate secondary antibodies and controls.
• The immunogen corresponds to the N-terminal region (aa 1–100); consider mapping the epitope if exact binding requirements are critical for your experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Annexin A4 antibody supports calcium-mediated membrane dynamics and secretion research. Whether you need validated antibody pairs, bulk monoclonal antibodies, or ancillary reagents, CamelBio’s supply chain delivers reliable, high-quality materials to accelerate your assay development.