Anti-Annexin A2 Monoclonal Antibody for WB, IHC-P, ELISA - P07355

Core Product Specifications and Parameters

Biological Background: Annexin A2 Function and Localization

• Annexin A2 is a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids; it binds two calcium ions with high affinity.
• It inhibits PCSK9-enhanced LDLR degradation, probably by reducing PCSK9 protein levels via a translational mechanism, and competes with LDLR for PCSK9 binding (PubMed:18799458, PubMed:22848640, PubMed:24808179).
  Related references: PMID:18799458 PMID:22848640 PMID:24808179
• The protein is required for endothelial cell surface plasmin generation and may support fibrinolytic surveillance and neoangiogenesis.
• It binds to damaged endosomes and participates in endosomal membrane stabilization, limiting NLRP3 inflammasome activation.
• In microbial infection, Annexin A2 acts as a receptor for M. pneumoniae CARDS toxin; its down-regulation reduces toxin binding and vacuolization.
• Subcellularly, it is found secreted into the extracellular space, associated with the basement membrane, and within melanosomes.
• Key annotations include annexin repeat, calcium/phospholipid-binding, phosphorylation, acetylation, ubiquitination, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 of human Annexin A2; the resulting monoclonal antibody may recognize epitopes within this N-terminal region.
• For Western blotting (WB), the predicted molecular weight is ~39 kDa; consider using appropriate molecular weight markers and validate detection in your sample system.
• In immunohistochemistry (IHC-P), antigen retrieval conditions and antibody dilution may need optimization for formalin-fixed paraffin-embedded tissue sections.
• For ELISA, use as a capture or detection antibody; pair with a matched antibody or validate against the recombinant protein.
• The antibody shows cross-reactivity with human, mouse, and rat samples; species-specific variations in target expression should be considered.
• Since Annexin A2 undergoes multiple post-translational modifications, verify that the antibody recognizes the relevant form under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for targets like Annexin A2. Whether your work involves coagulation, inflammation, or oncology, our integrated supply chain and consulting services support every stage from concept to clinic. Let CamelBio be your reliable B2B partner for high-quality raw materials and technical expertise.