Anti-Annexin A1/ANXA1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P04083

Core Product Specifications and Parameters

Biological Background: Annexin A1 Function and Localization

• Annexin A1, also known as Annexin I, Lipocortin I, Calpactin II, p35, and chromobindin-9, is a member of the annexin superfamily characterized by conserved calcium/phospholipid-binding repeats.
• It functions as an anti-inflammatory protein by mediating the effects of glucocorticoids and down-regulating the early phase of the inflammatory response (PubMed:8425544). Related references: PMID:8425544
• In adaptive immunity, Annexin A1 promotes the differentiation of T cells into Th1 cells while negatively regulating Th2 differentiation, thereby modulating T-cell activation and proliferation (PubMed:17008549). Related references: PMID:17008549
• The protein exhibits high-affinity Ca²⁺-dependent binding to phospholipid membranes and can bind up to eight Ca²⁺ ions, which is essential for its membrane association and function (PubMed:2532504; PubMed:8557678). Related references: PMID:2532504 PMID:8557678
• Annexin A1 signals through formyl peptide receptors (FPRs) to promote chemotaxis of granulocytes and monocytes, actin cytoskeleton rearrangement, cell polarization, and migration, and it plays a role in the resolution of inflammation and wound healing (PubMed:15187149; PubMed:22879591; PubMed:25664854). Related references: PMID:15187149 PMID:22879591 PMID:25664854
• It negatively regulates hormone exocytosis via FPR activation and actin reorganization (PubMed:19625660). Related references: PMID:19625660
• Subcellular localization is diverse, including the nucleus, cytoplasm, cell membrane, secretory vesicles, exosomes, and phagocytic cups, reflecting its multifunctional nature.
• Tissue expression is detected in resting neutrophils, peripheral blood T cells, placenta, liver, and extracellular vesicles from inflammatory bowel disease sera, consistent with its roles in immune and inflammatory processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2–346, covering nearly the full-length human Annexin A1 protein; this design often yields antibodies that recognize both native and denatured forms, making them suitable for multiple applications.
• For Western Blot (WB) and Immunohistochemistry (IHC), we recommend titrating the antibody in the relevant sample matrix to determine the optimal working concentration.
• In Immunofluorescence/Immunocytochemistry (IF/ICC), fixation and permeabilization conditions may need optimization due to Annexin A1’s presence in multiple subcellular compartments.
• The antibody has been verified for immunoprecipitation (IP) and ELISA; consider using appropriate positive controls such as stimulated immune cells or purified protein.
• Cross-reactivity with mouse and rat indicates conserved epitopes, but users should validate in their specific model systems.

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As a leading one-stop IVD raw-material provider, CamelBio ensures consistent supply of high-quality antibodies like this Annexin A1/ANXA1 monoclonal antibody, supporting your diagnostic development and immunological research. Our catalog includes validated antibody pairs, bulk ancillary reagents, and tailored sourcing services to accelerate your project from concept to clinic. Whether you need rare target raw materials or large-scale production support, CamelBio is your trusted bridge in the IVD supply chain.