Anti-Annexin A11 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P50995

Core Product Specifications and Parameters

Biological Background: Annexin A11 Function and Localization

• Annexin A11 is a member of the annexin family of calcium-dependent phospholipid-binding proteins, also known as 56 kDa autoantigen, Annexin XI, Annexin-11, or Calcyclin-associated annexin 50 (CAP-50).
• It specifically binds to calcyclin in a calcium-dependent manner, playing a role in calcium signaling and membrane-related processes.
• Annexin A11 is required for midbody formation and the completion of the terminal phase of cytokinesis, linking it to cell division.
• Subcellularly, it localizes to the cytoplasm, melanosome, nuclear envelope, nucleoplasm, and the cytoskeleton including the mitotic spindle.
• Post-translational modifications include acetylation, and alternative splicing yields multiple isoforms.
• Keywords associated with Annexin A11 include 3D-structure, amyotrophic lateral sclerosis (disease variant), neurodegeneration, cell cycle, cell division, and proteomics identification.
• It may have autoantigenic properties, as it was originally identified as a 56 kDa autoantigen.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 54 kDa; validate the antibody in your specific sample system using appropriate positive controls.
• When performing immunofluorescence or immunocytochemistry, consider optimizing fixation and permeabilization protocols to detect Annexin A11 in its various subcellular compartments (e.g., cytoplasm, nucleus, mitotic spindle).
• For immunoprecipitation, include proper negative controls (e.g., isotype-matched IgG) and confirm eluates by WB with a different anti-Annexin A11 antibody.
• In ELISA, titrate the antibody to determine optimal coating and detection conditions; use recombinant Annexin A11 or cell lysates as capture antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target procurement for Annexin A11 and related cell division, calcium signaling, and neurodegeneration research. Whether you require this polyclonal antibody or complementary raw materials for diagnostic assay development, our team can help bridge your concept-to-clinic needs.