Anti-Annexin A11 Monoclonal Antibody for WB, ELISA - P50995

Core Product Specifications and Parameters

Biological Background: Annexin A11 Function and Localization

• Annexin A11 is a member of the annexin family of calcium-dependent phospholipid-binding proteins.
• It specifically binds to calcyclin (S100A6) in a calcium-dependent manner (By similarity).
• Required for midbody formation and completion of the terminal phase of cytokinesis, playing a critical role in cell division.
• Localizes to the cytoplasm, melanosome, nuclear envelope, nucleoplasm, and cytoskeleton, including spindle structures.
• Post-translational modifications include acetylation; alternative splicing produces multiple isoforms.
• Associated with neurodegenerative disease; variants are linked to amyotrophic lateral sclerosis (ALS).

Experimental Guidance and Technical Tips

• The immunogen (aa 276–505) encompasses conserved annexin repeat domains; this may contribute to cross-reactivity observed across human and mouse species.
• For Western blotting, anticipate a specific band at approximately 54 kDa. Include appropriate positive and negative controls to confirm antibody specificity.
• For ELISA applications, the recombinant immunogen can serve as a reference standard to establish assay sensitivity and linearity.
• Given the protein's involvement in cytokinesis and cell division, consider testing in cell cycle-synchronized samples or mitotic cell fractions to enrich target detection.
• Validate antibody performance in the relevant sample system (e.g., HeLa cells for human, NIH/3T3 for mouse) under optimized experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined single-source solution for IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. This Annexin A11 antibody, targeting a key protein in cell division and neurodegenerative disease research, is part of CamelBio's extensive catalog supporting assay development and biomarker discovery. For specialized or rare target sourcing, our team provides tailored procurement support to accelerate your project timelines.