Anti-ANLN Rabbit Polyclonal Antibody for WB, ELISA - Q9NQW6

Core Product Specifications and Parameters

Biological Background: ANLN Function and Localization

• Anillin is an actin-binding protein that plays a critical role in cytokinesis; it is required for the structural integrity and completion of cleavage furrow ingression. Related references: PMID:16040610
• ANLN is involved in bleb assembly during metaphase and anaphase of mitosis, contributing to cell cortex dynamics. Related references: PMID:23870127
• It may significantly influence podocyte cell migration; expression is notably increased in renal biopsies from idiopathic focal segmental glomerulosclerosis (FSGS). Related references: PMID:24676636
• The protein localizes to multiple cellular compartments, including the nucleus, cytoplasm, cytoskeleton, cell cortex, and blebs, reflecting its dynamic role during cell division.
• Ubiquitously expressed with highest levels in brain, placenta, and testis; overexpression is observed in many tumor types (breast, colorectal, endometrial, hepatic, kidney, lung, ovarian, pancreatic).
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, regulating its function and localization.
• Alternative splicing generates multiple isoforms, providing functional diversity.
• Keywords associated with ANLN include Coiled coil, Actin-binding, Cell cycle, Cell division, Mitosis, indicating its structural and regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 845-1124) of human ANLN; this region may detect full-length protein and isoforms containing this domain.
• For Western blot, expect a band at ~124 kDa; use appropriate molecular weight markers and confirm specificity with relevant controls.
• In ELISA, the antibody may be used as capture or detection reagent; optimize coating and blocking conditions.
• ANLN is cell-cycle regulated; consider synchronizing proliferating cells to enhance detection.
• Cross-reactivity is limited to human; validate performance in human-derived samples.
• Optimal working dilutions must be determined empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For ANLN and cell division research, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our supply capabilities also include rare target raw-material sourcing to accelerate your development from concept to clinic.