Anti-ANKRD46 Rabbit Polyclonal Antibody - Q86W74

Core Product Specifications and Parameters

Biological Background: ANKRD46 Function and Localization

• ANKRD46 gene encodes the ankyrin repeat domain-containing protein 46, also known as ankyrin repeat small protein (ANK-S).
• The protein contains ankyrin repeat motifs, which are commonly involved in protein-protein interactions.
• Alternative splicing produces multiple transcript variants, contributing to isoform diversity (keyword: Alternative splicing).
• ANKRD46 localizes to the membrane, and is predicted to contain at least one transmembrane helix (keyword: Transmembrane, Transmembrane helix).
• The protein has been identified through high-throughput proteomics, supporting its expression in human cells (keyword: Proteomics identification).
• Its molecular weight, as determined by mass spectrometry, is around 25.3 kDa (Mol Weight: 25330 Da, consistent with the observed band).
• The precise biological function of ANKRD46 remains uncharacterized, but its ankyrin repeats and membrane association suggest roles in signal transduction or cellular architecture.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-232 of human ANKRD46 (NP_001257308.1), covering the full-length sequence of the predicted protein; this design may provide broad epitope recognition.
• For Western blot, the predicted molecular weight is approximately 25 kDa; however, post-translational modifications or alternative splicing could alter observed migration.
• In immunofluorescence and immunocytochemistry (IF/ICC), membrane localization is expected based on UniProt annotation; appropriate permeabilization and blocking steps should be optimized.
• The antibody demonstrates cross-reactivity with mouse and rat, as indicated by the supplier; nevertheless, users should validate reactivity in their specific experimental systems.
• For ELISA, the antibody can be employed as a capture or detection reagent after proper pair-screening and titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials, including validated antibodies like this ANKRD46 polyclonal antibody. With expertise in membrane proteins and rare targets, we support diagnostic developers and researchers with tailored sourcing solutions for polyclonal antibodies, recombinant proteins, and ancillary reagents. From concept to clinic, CamelBio bridges the gap between your project needs and reliable raw material supply.