Anti-ANKRD28 Rabbit Polyclonal Antibody for WB, ELISA - O15084

Core Product Specifications and Parameters

Biological Background: ANKRD28 Function and Localization

• ANKRD28, also known as Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A, PITK, FAP79, CFAP79, PPP1R65, is a regulatory subunit of protein phosphatase 6 (PP6) that mediates substrate recognition and targeting.
• It is involved in PP6-mediated dephosphorylation of NFKBIE (IκBε), opposing its TNF-induced degradation and thus modulating NF-κB signaling.
• ANKRD28 selectively inhibits the phosphatase activity of protein phosphatase 1 catalytic subunit (PPP1C) and targets PPP1C to modulate heterogeneous nuclear ribonucleoprotein K (HNRPK) phosphorylation.
• It participates in PP6-dependent dephosphorylation of MOB1, promoting focal adhesion assembly during cell migration. Related references: PMID:35512830
• The protein contains ankyrin repeat domains and is localized in the nucleoplasm, cytosol, and at lamellipodia, consistent with roles in both nuclear and cytosolic signaling.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, and the gene is subject to alternative promoter usage and splicing.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider testing a dilution range of 1:500 to 1:2000 and validate detection of endogenous ANKRD28 in relevant cell lysates (e.g., HeLa, HEK293). The predicted molecular weight is 113 kDa, but observe for possible post-translational modifications or splice isoforms.
• For ELISA, pair this antibody with a matched detection reagent and titrate from 0.1–1.0 µg/mL; include appropriate positive and negative controls.
• The immunogen spans the N-terminal 300 amino acids; so the antibody may recognize multiple isoforms. Verify reactivity in your specific experimental system.
• This antibody cross-reacts with human and mouse; when using mouse samples, perform a species-specific validation to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk reagents to support phosphatase signaling and cell migration research. Whether you need this ANKRD28 antibody as part of a kit or require tailored raw-material supply, our team can help source rare targets and scale production efficiently. Contact us to discuss your diagnostic development needs from concept to clinic.