Anti-ANKRD1 Rabbit PolymAb® for WB and ELISA - Q15327

Core Product Specifications and Parameters

Biological Background: ANKRD1 Function and Localization

• ANKRD1 (Ankyrin repeat domain-containing protein 1), also known as Cardiac ankyrin repeat protein (CARP) or Cytokine-inducible gene C-193 protein, is a member of the ankyrin repeat protein family.
• It functions as a nuclear transcription factor that negatively regulates the expression of cardiac genes, thus playing a role in heart development and disease.
• ANKRD1 may also be involved in endothelial cell activation, indicating a role in vascular biology.
• Its expression is induced in response to cytokine stimulation and has been correlated with apoptotic cell death in hepatoma cells.
• The protein localizes to the nucleus, consistent with its transcriptional regulatory activity.
• Mainly expressed in activated vascular endothelial cells, with lower expression in hepatoma cells.
• Contains ANK repeat motifs and a coiled-coil domain, facilitating protein-protein interactions.

Experimental Guidance and Technical Tips

• For Western blot analysis, ANKRD1 migrates at an apparent molecular weight of 36 kDa; use appropriate gel percentage and transfer conditions.
• Nuclear localization requires efficient nuclear extraction protocols for optimal detection; consider subcellular fractionation or high-salt lysis buffers.
• Due to low basal expression in many cell types, induction with cytokines (e.g., TNF-α, IL-1β) or use of disease-state tissues may enhance signal.
• Cross-reactivity with mouse and rat ANKRD1 enables translational studies in rodent models; verify reactivity in the specific tissue or cell line of interest.
• For ELISA, pairing with a validated capture antibody may improve sensitivity; validate the system using recombinant ANKRD1 protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutions with a comprehensive range of IVD raw materials, technical services, and consulting support. For ANKRD1 research in cardiovascular biology, endothelial activation, or apoptosis, we supply validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development from concept to clinic.