Anti-ANKRD1 Rabbit Polyclonal Antibody for WB and ELISA - Q15327

Core Product Specifications and Parameters

Biological Background: ANKRD1 Function and Localization

• ANKRD1, also known as cardiac ankyrin repeat protein (CARP) and cytokine-inducible gene C-193 protein, is characterized by ankyrin repeat and coiled-coil domains.
• It may function in endothelial cell activation and as a nuclear transcription factor that negatively regulates cardiac gene expression.
• Induction of ANKRD1 has been correlated with apoptotic cell death in hepatoma cells.
• Localized primarily to the nucleus.
• Mainly expressed in activated vascular endothelial cells, with lower expression in hepatoma cells.
• Contains ankyrin repeat motifs and a coiled-coil region, features typical of transcriptional regulators.

Experimental Guidance and Technical Tips

• The immunogen sequence covers the N-terminal 130 amino acids, a region that includes predicted ankyrin repeat motifs; thus, the antibody may detect full-length ANKRD1 (~36 kDa) on Western blots.
• For Western blot, consider preparing nuclear extracts to enrich the target protein, given its nuclear localization.
• In ELISA, optimize coating antigen concentrations and antibody dilutions using recombinant ANKRD1 or cell lysates as positive controls.
• Validate cross-reactivity in mouse and rat samples (e.g., endothelial or hepatoma cell lines) to confirm binding.
• Include appropriate negative controls (e.g., non-transfected cells or tissues with low ANKRD1 expression) to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to validated IVD raw materials for cardiovascular and endothelial biology research. Our supply capabilities include optimized polyclonal antibodies like this ANKRD1 antibody, suitable for WB and ELISA applications. Complement your projects with our bulk ancillary reagents and custom rare target sourcing services to accelerate diagnostic development.