Anti-ANKRD1 Monoclonal Antibody for WB, ELISA - Q15327

Core Product Specifications and Parameters

Biological Background: ANKRD1 Function and Localization

• ANKRD1, also known as cardiac ankyrin repeat protein (CARP) or cytokine-inducible gene C-193 protein, is a member of the ankyrin repeat domain-containing protein family.
• It functions as a nuclear transcription factor that negatively regulates the expression of cardiac genes, suggesting a role in heart development and disease.
• ANKRD1 may play an important role in endothelial cell activation, contributing to vascular biology and inflammation.
• Its induction has been correlated with apoptotic cell death in hepatoma cells, indicating involvement in programmed cell death pathways.
• The protein is predominantly localized to the nucleus.
• It is mainly expressed in activated vascular endothelial cells, with lower expression in hepatoma cells.
• Key structural features include ankyrin repeats and a coiled coil domain, which may mediate protein-protein interactions.

Experimental Guidance and Technical Tips

• For Western blot, ANKRD1 has a predicted molecular weight of approximately 36 kDa; consider using reducing and denaturing gel conditions and validate detection in your specific sample lysate.
• For ELISA applications, titrate the antibody to establish the optimal working concentration for your assay format, considering both direct and sandwich ELISA configurations.
• This antibody cross-reacts with human and mouse ANKRD1; confirm species-specific reactivity and signal specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, providing diagnostic manufacturers and laboratories with validated antibodies, antigen pairs, and related reagents. Our ANKRD1 monoclonal antibody supports research into cardiovascular disease, endothelial function, and apoptosis, and we can supply bulk quantities with consistent lot-to-lot performance. For custom sourcing or technical consultation on rare targets, CamelBio offers end-to-end support from concept to clinic.