Anti-ANKHD1 Polyclonal Antibody for WB, ELISA - Q8IWZ3

Core Product Specifications and Parameters

Biological Background: ANKHD1 Function and Localization

• ANKHD1 (Ankyrin repeat and KH domain-containing protein 1), also known as MASK (Multiple ankyrin repeats single KH domain) and HIV-1 Vpr-binding ankyrin repeat protein, is encoded by the ANKHD1 gene (GeneID: 54882) and characterized by multiple ankyrin repeats and a KH domain, suggesting roles in protein-protein interactions and potential RNA binding.
• The protein is primarily localized in the cytoplasm and functions as a scaffolding protein, possibly contributing to the abnormal phenotype observed in leukemia cells.
• Isoform 2 exhibits an antiapoptotic effect by regulating caspases, thereby protecting cells during normal survival; this highlights its potential involvement in apoptosis modulation.
• ANKHD1 is ubiquitously expressed, with relatively higher levels in the cervix, spleen, and brain; its expression is notably elevated in leukemia cells, and isoform 2 is abundant in the spleen but nearly absent in muscle and brain.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, and alternative splicing generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western blot detection, ANKHD1 has a predicted molecular weight of 269 kDa; consider using a low-percentage acrylamide gel (e.g., 6–8%) and extended transfer times to ensure efficient transfer of high-molecular-weight proteins.
• The immunogen corresponds to amino acids 1620–1800 within the C-terminal region; this polyclonal antibody is expected to recognize full-length ANKHD1 and possibly its isoforms, but validation in the relevant sample system is recommended.
• In ELISA applications, validate the antibody using recombinant ANKHD1 protein or lysates from human cell lines known to express ANKHD1 (e.g., leukemia-derived lines) to confirm specificity and sensitivity.
• Given the ubiquitous but variable expression, include appropriate positive and negative controls (e.g., spleen vs. muscle lysates) to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, technical services, and consulting, supporting research and diagnostic development from concept to clinic. This ANKHD1 antibody is positioned for applications in leukemia and apoptosis research, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw material sourcing to streamline your workflow.