Anti-ANKH Rabbit Polyclonal Antibody for WB, ELISA - Q9HCJ1

Core Product Specifications and Parameters

Biological Background: ANKH Function and Localization

• ANKH is a transmembrane protein that functions as an ATP and NTP transporter, primarily localized to the cell membrane.
• By exporting NTPs, ANKH provides substrates for ENPP1 to generate extracellular inorganic pyrophosphate (PPi), a key calcification inhibitor, thereby preventing abnormal joint mineralization. Related references: PMID:20943778, PMID:32639996, PMID:35147247
• ANKH also mediates the release of TCA cycle intermediates (citrate, succinate, malate) to the extracellular space, where citrate supports osteogenic differentiation and bone strength. Related references: PMID:32639996
• The protein is predominantly expressed in osteoblasts from mandibular and iliac bone, consistent with its role in bone mineralization.
• Alternative splicing produces multiple isoforms, and genetic variants in ANKH are linked to craniometaphyseal dysplasia, chondrocalcinosis, and deafness (UniProt keywords: Alternative splicing, Disease variant, Deafness).

Experimental Guidance and Technical Tips

• The immunogen targets a central region (aa 210-320) of human ANKH, which may recognize multiple isoforms depending on epitope conservation.
• For Western blot analysis, consider using positive control lysates from osteoblast cell lines (e.g., human osteosarcoma) given the tissue expression profile.
• For ELISA applications, verify antibody reactivity with recombinant ANKH protein and consider pairing with a detection antibody to improve sensitivity.
• The predicted molecular weight is 54 kDa; post-translational modifications may cause migration shifts.
• Cross-reactivity is reported for human; validate in other species if required for your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. With a focus on bone and mineral metabolism targets like ANKH, we offer one-stop sourcing for rare and specialized reagents. Our supply solutions cover everything from early-stage R&D to large-scale production, backed by technical expertise.