Anti-ANGPTL5 Rabbit Polyclonal Antibody for WB, ELISA - Q86XS5

Core Product Specifications and Parameters

Biological Background: ANGPTL5 Function and Localization

• ANGPTL5, also known as Angiopoietin-like protein 5, is a member of the angiopoietin-related protein family characterized by a coiled-coil domain and structural similarity to angiopoietins.
• The protein is secreted and contains a signal peptide, indicating extracellular localization and a role in intercellular signaling.
• Disulfide bond formation is predicted, suggesting a structurally stabilized secreted protein.
• Annotation as a glycoprotein implies post-translational glycosylation, which may influence protein folding, stability, or receptor interactions.
• Mainly expressed in adult heart, pointing to a potential function in cardiac physiology or pathology.
• The precise biological function of ANGPTL5 remains to be fully elucidated, and it is identified through proteomics approaches as part of the human reference proteome.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, consider using human cardiac tissue lysates or recombinant ANGPTL5 protein as positive controls to verify antibody specificity.
• Optimize blocking and antibody dilution conditions empirically, as polyclonal antibodies may require titration to reduce nonspecific binding.
• In ELISA applications, coat with recombinant ANGPTL5 protein and validate with known concentrations to establish reliable standard curves.
• Since the immunogen covers the full-length protein (amino acids 1-388), the antibody may recognize both native and denatured forms; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing bulk ancillary reagents, validated antibody pairs, and a comprehensive range of monoclonal and polyclonal antibodies. For research on angiopoietin-related biology and cardiac biomarkers, we facilitate access to rare target raw materials and offer tailored support from concept to clinic. Streamline your cardiovascular research reagent procurement with CamelBio.