Anti-ANGPTL3 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9Y5C1

Core Product Specifications and Parameters

Biological Background: ANGPTL3 Function and Localization

• ANGPTL3 (angiopoietin-related protein 3, also known as ANG-5 or ANGPT5) is a secreted glycoprotein that belongs to the angiopoietin-like family, primarily functioning as a hepatokine regulating systemic lipid and glucose homeostasis.
• It stimulates plasma triglyceride levels by suppressing lipoprotein lipase (LPL) activity through an indirect mechanism that involves proprotein convertases PCSK6 and FURIN, leading to cleavage and inactivation of LPL on endothelial surfaces. Related references: PMID:12097324, PMID:19318355, PMID:20581395
• ANGPTL3 also inhibits endothelial lipase, elevating high-density lipoprotein (HDL) cholesterol and phospholipid levels. Related references: PMID:17110602, PMID:19028676
• In adipocytes, it can bind and trigger lipolysis, increasing free fatty acids and glycerol release. Related references: PMID:12565906
• Beyond lipid metabolism, ANGPTL3 plays a role in angiogenesis by binding to endothelial cells via integrin αvβ3, activating FAK, MAPK, and Akt signaling, which promotes cell adhesion and migration. Related references: PMID:11877390, PMID:18535744
• The protein is secreted and localizes to the lamellipodium/cell projection regions, consistent with its roles in cell motility and adhesion.
• It is predominantly expressed in the liver, with weaker expression in the kidney, and its increased glomerular expression correlates with nephrotic syndrome.
• Post-translational modifications include glycosylation and disulfide bond formation; it features a coiled-coil domain and a heparin-binding motif.

Experimental Guidance and Technical Tips

• Immunogen design: The synthetic peptide from the N-terminal region (aa 1‑100) encompasses motifs critical for LPL interaction; the antibody may recognize both full‑length ANGPTL3 (~54 kDa) and N‑terminal fragments.
• Western blot (WB): Use reducing conditions and consider that glycosylation may cause migration shifts; validate in liver lysates (human, mouse, rat) for expected bands.
• Immunofluorescence/immunohistochemistry (IF‑P, IHC‑P): Permeabilization is recommended for intracellular detection; note potential localization to lamellipodia in endothelial cells and podocytes.
• ELISA: This monoclonal antibody is suitable for capture or detection; pair with a different clone or polyclonal for optimal sandwich ELISA performance.
• Cross‑reactivity: Confirmed with human, mouse, and rat; sequence alignment suggests reactivity with conserved N‑terminal regions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop IVD raw‑material sourcing partner, offering this anti‑ANGPTL3 rabbit monoclonal antibody along with validated antibody pairs, optimized bulk reagents, and rare‑target sourcing for lipid metabolism, cardiovascular, and angiogenesis assay development. Our integrated supply chain supports diagnostic manufacturers and researchers from concept to clinic, ensuring reliable access to critical raw materials.