Anti-ANGPTL3 Polyclonal Antibody for WB, ELISA - Q9Y5C1

Core Product Specifications and Parameters

Biological Background: ANGPTL3 Function and Localization

• ANGPTL3, also known as angiopoietin-5, is a member of the angiopoietin-like protein family encoded by the ANGPTL3 gene in humans.
• It acts as a liver-derived hepatokine with a central role in the regulation of systemic lipid and glucose metabolism. Related references: PMID:11788823, PMID:12909640, PMID:23661675
• ANGPTL3 significantly elevates plasma triglyceride levels by inhibiting lipoprotein lipase (LPL) activity through the recruitment of proprotein convertases PCSK6 and FURIN, which cleave and release LPL from the cell surface; this function is mediated by the N-terminal domain and does not require ANGPTL3 cleavage. Related references: PMID:12097324, PMID:19318355, PMID:20581395
• It also inhibits endothelial lipase, leading to increased plasma levels of high-density lipoprotein (HDL) cholesterol and phospholipids. Related references: PMID:17110602, PMID:19028676
• ANGPTL3 is involved in angiogenesis: it binds to endothelial cells via integrin alpha-V/beta-3, activates FAK, MAPK, and Akt signaling pathways, and promotes cell adhesion and migration. Additionally, secreted from podocytes, it may modulate glomerular endothelial cell properties. Related references: PMID:11877390, PMID:18535744
• The protein is secreted and localizes to lamellipodia. It is predominantly expressed in the liver, with weak expression in the kidney, and binds to adipocytes; its expression is increased in glomeruli of nephrotic syndrome patients.
• ANGPTL3 is a glycoprotein with disulfide bonds and contains coiled-coil and heparin-binding domains.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 231–460, covering the C-terminal fibrinogen-like domain. This region may contribute to specific detection of full-length ANGPTL3.
• For Western blot analysis, a band at approximately 54 kDa is expected; consider using reducing conditions and appropriate controls.
• The antibody shows cross-reactivity with mouse ANGPTL3, making it suitable for studies in mouse models; validate in the relevant tissue or cell lysates.
• For ELISA applications, optimize coating conditions and antibody dilution to achieve desired sensitivity and specificity.
• Because ANGPTL3 is a secreted hepatokine, consider its presence in serum or plasma samples when designing experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic manufacturers from concept to clinic. Our portfolio includes optimized polyclonal antibodies like this ANGPTL3 antibody, tailored for applications in lipid metabolism and angiogenesis research. We also provide validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your assay development.