Anti-ANGPT2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O15123

Core Product Specifications and Parameters

Biological Background: ANGPT2 Function and Localization

• Angiopoietin-2 (ANGPT2), also known as ANG2, AGPT2, or LMPHM10, is a secreted glycoprotein belonging to the angiopoietin family of growth factors.
• It binds the TIE2 receptor, competing with ANGPT1 and modulating downstream signaling; in the absence of ANGPT1, ANGPT2 can induce TIE2 tyrosine phosphorylation. Related references: PMID:15284220 PMID:19116766 PMID:19223473
• In environments lacking VEGF, ANGPT2-mediated disruption of endothelial cell–matrix contacts may promote apoptosis and vascular regression. Related references: PMID:15284220 PMID:19116766 PMID:19223473
• Conversely, in the presence of VEGF, ANGPT2 acts as a permissive angiogenic signal by facilitating endothelial cell migration and proliferation. Related references: PMID:19116766 PMID:19223473
• ANGPT2 is also implicated in lymphangiogenesis. Related references: PMID:32908006
• Subcellularly, ANGPT2 is secreted and contains conserved structural motifs including a coiled-coil domain, disulfide bonds, and calcium-binding sites.
• Post‑translational modifications such as glycosylation and disulfide bond formation are essential for proper folding and stability; alternative splicing generates multiple isoforms.
• ANGPT2 is associated with disease variants and participates in developmental and differentiation processes.

Experimental Guidance and Technical Tips

• The immunogen (a peptide spanning residues 300–400 of human ANGPT2) is designed from the central region; verify antibody binding to full‑length endogenous protein in your sample system.
• For Western blotting, ANGPT2 migrates at approximately 57 kDa; use an appropriate gel percentage and membrane transfer conditions.
• For immunohistochemistry (IHC‑P), consider antigen retrieval protocols and include appropriate controls to confirm specificity.
• In ELISA, validate linearity and matrix effects using recombinant ANGPT2 or relevant biological fluids.
• Cross‑reactivity with mouse and rat allows application in rodent angiogenesis models; confirm reactivity under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target procurement. This ANGPT2 antibody exemplifies our commitment to providing high-quality reagents for angiogenesis and cardiovascular disease research. From concept to clinic, CamelBio bridges your diagnostic development needs.