Anti-ANGPT2 Polyclonal Antibody for WB, IF/ICC, ELISA - O15123

Core Product Specifications and Parameters

Biological Background: Angiopoietin-2 Function and Localization

• ANGPT2, also known as ANG2, is a secreted glycoprotein that serves as a context-dependent regulator of angiogenesis, binding to the TIE2 receptor.
• It competes with ANGPT1 for TIE2 binding, modulating downstream signaling; in the absence of VEGF, ANGPT2 promotes vascular regression by inducing endothelial cell apoptosis, while in the presence of VEGF it facilitates endothelial migration and proliferation, acting as a permissive angiogenic signal. Related references: PMID:15284220 PMID:19116766 PMID:19223473
• ANGPT2 is also involved in the regulation of lymphangiogenesis. Related references: PMID:32908006
• It is a secreted protein, synthesized with a signal peptide, and is found in the extracellular space.
• The protein is post-translationally modified with glycosylation and disulfide bonds, and its structure includes a coiled-coil domain; alternative splicing generates multiple isoforms.
• UniProt keywords include Angiogenesis, Developmental protein, Disease variant, Glycoprotein, Secreted, and 3D-structure, underscoring its role in vascular development and pathology.
• The predicted molecular weight is approximately 57 kDa.

Experimental Guidance and Technical Tips

• For Western blot (WB), run samples under reducing conditions and validate that the primary band appears near 57 kDa; additional bands may correspond to glycosylated variants or isoforms.
• In immunofluorescence (IF/ICC), consider that ANGPT2 is primarily secreted; intracellular staining may require permeabilization and inclusion of secretion inhibitors (e.g., brefeldin A) to visualize the intracellular pool.
• For ELISA, this polyclonal antibody may be used either as a capture or detection reagent after appropriate titration and pairing validation.
• Given cross-reactivity with mouse and rat, validate the antibody in your specific model system and optimize blocking/dilution conditions to minimize species-specific background.
• The immunogen covers amino acids 197–496, which is the central portion of the protein; it is expected to recognize all major isoforms, but confirm reactivity for your target isoform if isoform-specific detection is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets in angiogenesis and vascular biology. Partner with us to streamline your ANGPT2 assay development and secure reliable, high-quality raw materials.