Anti-ANGPT1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15389

Core Product Specifications and Parameters

Biological Background: Angiopoietin-1 Function and Localization

• Angiopoietin-1 (ANGPT1) is a secreted glycoprotein that serves as a ligand for the TEK/TIE2 receptor tyrosine kinase, playing a central role in vascular development and maintenance.
• Upon binding, ANGPT1 induces TEK dimerization and tyrosine phosphorylation, activating downstream signaling cascades that regulate endothelial cell survival, proliferation, migration, adhesion, and actin cytoskeleton reorganization.
• During embryogenesis, ANGPT1 is essential for normal angiogenesis and heart development, acting later and distinctly from VEGF-mediated pathways.
• In quiescent, confluent endothelial cells, ANGPT1 oligomers recruit TEK to cell-cell junctions, preferentially activating the PI3K-AKT pathway to promote vascular stability and inhibit angiogenesis.
• In migrating endothelial cells lacking cell-cell contacts, ANGPT1 anchors TEK to the extracellular matrix, stimulating focal adhesion kinase (FAK) and MAPK/ERK signaling to drive sprouting angiogenesis.
• ANGPT1 mediates blood vessel maturation and stabilization, and is implicated in reciprocal interactions between the endothelium and surrounding mesenchyme.
• The protein undergoes alternative splicing, and the canonical isoform contains a signal peptide, coiled-coil domains, and a C-terminal fibrinogen-like domain; it is subject to disulfide bond formation and glycosylation.
• ANGPT1 is associated with disease variants (e.g., HAE5) and is a reference proteome member; it is secreted into the extracellular space.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 277–498 of human ANGPT1, which spans the fibrinogen-like domain. This region is conserved in mouse and rat, consistent with the antibody's reported cross-reactivity. For Western blotting, anticipate a band near 58 kDa under reducing conditions; lysates from endothelial-rich tissues (e.g., lung, placenta) may serve as positive controls.
• For immunohistochemistry (IHC-P), antigen retrieval (e.g., heat-induced epitope retrieval in citrate buffer) may improve staining. Endogenous ANGPT1 expression is often low; consider amplifying signal with sensitive detection systems.
• In ELISA, the antibody can be used as a capture or detection reagent; pairing with a validated monoclonal antibody may enhance specificity. Validate assay conditions in the relevant sample matrix.
• The antibody recognizes both endogenous and recombinant ANGPT1. When using recombinant protein as a standard, ensure it covers the immunogen sequence to verify antibody reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. This anti-ANGPT1 rabbit polyclonal antibody is well-suited for research into angiogenesis, vascular biology, and related disorders. Whether you need bulk polyclonal antibodies, optimized monoclonal pairs, or custom sourcing for rare targets, CamelBio bridges the gap between research needs and supply chain scalability.