Anti-ANGPT1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q15389

Core Product Specifications and Parameters

Biological Background: ANGPT1 Function and Localization

• ANGPT1, also known as AGP1, AGPT, ANG1, or HAE5, is a member of the angiopoietin family. It binds and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation.
• It regulates angiogenesis by promoting endothelial cell survival, proliferation, migration, adhesion, and cytoskeletal reorganization. The protein exhibits context-dependent effects, inhibiting angiogenesis in quiescent vessels while stimulating sprouting angiogenesis in migrating endothelial cells.
• ANGPT1 is essential for normal angiogenesis and heart development during embryogenesis, and postnatally it mediates blood vessel maturation and stability, maintaining vascular quiescence.
• It facilitates reciprocal interactions between the endothelium and the surrounding matrix and mesenchyme, and is implicated in developmental processes distinct from VEGF.
• Subcellularly, ANGPT1 is secreted and acts extracellularly. It undergoes post-translational modifications including glycosylation and formation of disulfide bonds, and may exist as coiled-coil oligomers.
• Alternative splicing results in multiple transcript variants, and the protein is associated with disease variants (keyword), though specific pathogenic roles are not detailed.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 200–300 of human ANGPT1 (UniProt Q15389). For Western blotting, anticipate a band at approximately 58 kDa under reducing conditions; validate using a relevant positive control lysate from human, mouse, or rat endothelial cells.
• For immunohistochemistry (IHC-P), consider performing antigen retrieval to enhance epitope exposure. Validate antibody specificity using tissue sections with known high ANGPT1 expression, such as placental or tumor vasculature.
• In ELISA applications, the antibody can be used as a detection reagent; pairing with an appropriate capture antibody may be necessary for sandwich ELISA. Titrate the antibody concentration for optimal signal-to-noise ratio.
• The antibody shows cross-reactivity with mouse and rat ANGPT1, making it suitable for multi-species studies; however, always confirm performance in the target species and application system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-ANGPT1 rabbit monoclonal antibody exemplifies our commitment to providing high-quality reagents for angiogenesis, cardiovascular disease, and cancer research. Whether you require validated antibody pairs, bulk monoclonal or polyclonal antibodies, or rare target raw materials, CamelBio streamlines your procurement with technical expertise and reliable supply chains.