Anti-Angiotensinogen Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P01019

Core Product Specifications and Parameters

Biological Background: Angiotensinogen Function and Localization

• Angiotensinogen, also known as Serpin A8, is the precursor of angiotensin peptides and a key component of the renin-angiotensin system (RAS), regulating blood pressure, fluid and electrolyte balance (PubMed:10619573, PubMed:17138938). Related references: PMID:10619573 PMID:17138938
• It exerts vasoconstrictor effects directly on vascular smooth muscle and influences cardiac contractility, heart rate, and renal sodium/water absorption via aldosterone stimulation (PubMed:10619573, PubMed:17138938). Related references: PMID:10619573 PMID:17138938
• Acts as a ligand for angiotensin receptors AGTR1 and AGTR2, mediating its classical pressor actions (PubMed:1567413). Related references: PMID:1567413
• Additionally, angiotensinogen-derived peptides bind the MAS1 receptor, promoting vasodilation, antidiuresis, and antithrombotic effects through nitric oxide release (By similarity).
• The protein is primarily expressed by hepatocytes in the liver and secreted into the plasma (Secreted).
• It belongs to the serpin family and contains a signal peptide, multiple disulfide bonds, and glycosylation sites (Keywords: Disulfide bond, Glycoprotein).
• Genetic variations in AGT are associated with disorders of blood pressure homeostasis (Keywords: Disease variant).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature protein (lacking signal peptide); the antibody likely recognizes both precursor and mature forms—consider verifying in relevant sample systems.
• For western blotting, the predicted molecular weight is ~53 kDa (variable due to glycosylation); validate with proper controls.
• For immunohistochemistry (IHC) and immunofluorescence (IF), note that angiotensinogen is secreted, so signal may appear in extracellular spaces or intracellular compartments depending on fixation and permeabilization; optimize protocols accordingly.
• This polyclonal antibody may be suitable for ELISA-based quantification of angiotensinogen in plasma or serum; consider pairing with appropriate standards.
• Cross-reactivity with mouse and rat suggests utility in common animal models; however, sequence homology should be checked for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical services to support diagnostic development from concept to clinic. This anti-Angiotensinogen antibody enables research into the renin-angiotensin system, hypertension, and cardiovascular disorders. Our sourcing platform provides access to validated antibody pairs, bulk ancillary reagents, and rare target raw materials to streamline your assay development.