Anti-Angiotensinogen Rabbit Monoclonal Antibody for WB, ELISA - P01019

Core Product Specifications and Parameters

Biological Background: Angiotensinogen Function and Localization

• Angiotensinogen (Serpin A8) is an essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid, and electrolyte homeostasis. It acts directly on vascular smooth muscle as a potent vasoconstrictor and stimulates aldosterone secretion from the adrenal cortex (PubMed:10619573, PubMed:17138938). Related references: PMID:10619573, PMID:17138938
• The protein signals through binding to angiotensin receptors AGTR1 and AGTR2, mediating its effects on vascular tone and cardiac function (PubMed:1567413). Related references: PMID:1567413
• Angiotensinogen also serves as a ligand for the MAS1 receptor, exhibiting vasodilator, antidiuretic, and antithrombotic activities (By similarity).
• It is a secreted glycoprotein with a signal peptide and several disulfide bonds that stabilize its structure.
• Angiotensinogen is predominantly expressed by the liver and released into the plasma, consistent with its endocrine role.
• As a member of the serpin (serine protease inhibitor) superfamily, angiotensinogen shares structural features but primarily functions as a hormone precursor.
• The protein has a molecular weight of approximately 52 kDa from sequence but migrates at about 53 kDa in SDS-PAGE due to glycosylation.
• Keywords derived from UniProt include: Vasoactive, Vasoconstrictor, Secreted, Glycoprotein, Disulfide bond, Disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human angiotensinogen, suggesting the antibody may recognize an epitope within this domain.
• For Western blot, a band around 53 kDa is expected; consider reducing conditions and a low-percentage gel for optimal resolution.
• The antibody cross-reacts with mouse and rat angiotensinogen, enabling its use in preclinical rodent models where the renin-angiotensin system is studied.
• In ELISA applications, validate antibody specificity using recombinant angiotensinogen protein or peptide blocking controls.
• No other applications are listed; for IHC or ICC, antigen retrieval optimization and primary antibody titration are recommended if exploring additional uses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-angiotensinogen antibody supports research into hypertension, cardiovascular disease, and renal function, and CamelBio streamlines procurement across the entire IVD development cycle.