Anti-ANG (Angiogenin) Rabbit Polyclonal Antibody for WB and ELISA - P03950

Core Product Specifications and Parameters

Biological Background: Angiogenin Function and Localization

• Angiogenin (ANG, also known as RNase5) is a secreted ribonuclease that can either promote or restrict cell proliferation depending on the biological context (PubMed:12051708, PubMed:1400510, PubMed:19332886). Related references: PMID:12051708, PMID:1400510, PMID:19332886

• Under stress conditions, ANG localizes to stress granules and cleaves a subset of tRNAs (e.g., tRNA(Ala), tRNA(Glu), tRNA(Gly), etc.) within anticodon loops, preferentially between pyrimidine and adenosine residues, to produce tRNA-derived stress-induced fragments (tiRNAs); this results in translation repression and inhibition of apoptosis (PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:3289612, PubMed:38718836, PubMed:31582561; apoptosis inhibition by similarity). Related references: PMID:1400510, PMID:19332886, PMID:20129916, PMID:3289612, PMID:38718836, PMID:31582561

• Under growth conditions, ANG translocates to the nucleus and nucleolus, where it stimulates rRNA transcription, promoting cell growth, proliferation, and angiogenesis (PubMed:12051708, PubMed:15735021, PubMed:27518564, PubMed:19354288, PubMed:4074709, PubMed:8448182). Related references: PMID:12051708, PMID:15735021, PMID:27518564, PMID:19354288, PMID:4074709, PMID:8448182

• ANG is critical for hematopoietic stem and progenitor cell (HSPC) growth and survival, balancing rRNA transcription and translation inhibition in response to environmental cues (PubMed:27518564). Related references: PMID:27518564

• It mediates crosstalk between myeloid and intestinal epithelial cells, protecting the intestinal epithelial barrier integrity (PubMed:32510170). Related references: PMID:32510170

• Subcellular localization includes secreted form, cytoplasm, nucleus, nucleolus, and stress granules.

• Predominantly expressed in the liver, with additional expression in endothelial cells and spinal cord neurons (PubMed:2440105, PubMed:17886298). Related references: PMID:2440105, PMID:17886298

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight is approximately 17 kDa. Consider using reducing conditions and validate detection in human and rat cell lysates or tissue homogenates.
• For ELISA applications, the antibody can be used as a capture or detection reagent; determine optimal coating concentrations and incubation conditions empirically using the recombinant immunogen as a reference standard.
• As a polyclonal antibody, lot-to-lot consistency should be verified for your specific assay requirements. Validate the antibody's performance in your experimental system before large-scale studies.
• Given ANG's secretion, consider testing conditioned medium or serum samples in addition to cell lysates for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinical application. For angiogenesis and stress response research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Our global supply chain also enables sourcing of rare target raw materials, ensuring you have uninterrupted access to critical components.