Anti-Androgen Receptor Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P10275

Core Product Specifications and Parameters

Biological Background: Androgen Receptor Function and Localization

• The androgen receptor (AR) functions as a ligand-activated transcription factor that regulates gene expression, influencing cellular proliferation and differentiation in target tissues. Related references: PMID:19022849
• AR transcriptional activity is modulated by coactivators and corepressors such as ZBTB7A, which recruits NCOR1/NCOR2 to androgen response elements, thereby inhibiting AR signaling and androgen-induced cell proliferation. Related references: PMID:20812024
• Alternative splicing produces isoform 3, which lacks the C-terminal ligand-binding domain and constitutively activates transcription of a specific set of canonical AR-target genes (e.g., PSA/KLK3, TMPRSS2) independently of steroid hormones. Related references: PMID:19244107, PMID:25008967
• AR localizes to both the nucleus and cytoplasm, with ligand binding inducing nuclear translocation and transcriptional activation.
• At the protein level, AR is predominantly expressed in heart and skeletal muscle, with additional expression in basal and stromal cells of the prostate.
• AR undergoes multiple post-translational modifications including phosphorylation, palmitoylation, and ubiquitination, which regulate its stability, transcriptional activity, and subcellular localization.
• Mutations and triplet repeat expansions in the AR gene are implicated in androgen insensitivity syndrome, spinal and bulbar muscular atrophy (SBMA), and prostate cancer.
• AR is a member of the nuclear receptor subfamily 3 group C (NR3C4) and contains zinc-finger DNA-binding domains that enable interaction with specific androgen response elements.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human AR, which is present in both full-length and N-terminal isoforms. Confirm detection of specific isoforms in your model system.
• For western blotting, the predicted molecular weight of AR is approximately 99 kDa. Nuclear or whole-cell lysates may be suitable; validate using appropriate controls.
• In immunofluorescence/ICC, consider optimizing fixation and permeabilization protocols to detect both nuclear and cytoplasmic AR pools.
• Cross-reactivity with mouse and rat AR is indicated; however, you may wish to verify by sequence alignment or tissue-specific expression patterns.
• ELISA can be employed for antibody titer determination or screening assays; establish optimal coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials, including validated anti-androgen receptor antibodies and related nuclear receptor targets. Our comprehensive supply chain covers monoclonal and polyclonal antibodies, bulk reagents, and customized sourcing for rare or challenging targets, supporting your development from concept to clinic. With this androgen receptor antibody and our full portfolio, you can accelerate your next-generation assays and research initiatives.