Anti-Androgen Receptor Monoclonal Antibody for WB, IP, IHC-P, mIHC, IF-P, ELISA - P10275

Core Product Specifications and Parameters

Biological Background: Androgen Receptor Function and Localization

• The androgen receptor is a ligand-activated transcription factor belonging to the nuclear receptor superfamily. It regulates gene expression by binding to androgen response elements, thereby influencing cell proliferation and differentiation. Related references: PMID:19022849
• Transcriptional activity is modulated by coactivators and corepressors, such as ZBTB7A, which recruits NCOR1 and NCOR2 to repress androgen-induced cell proliferation. Related references: PMID:20812024
• Alternative splicing produces multiple isoforms, including a full-length receptor (isoform 1) and isoform 3, which lacks the C-terminal ligand-binding domain and constitutively regulates a distinct set of target genes independently of steroid hormones. Related references: PMID:19244107 PMID:25008967
• The receptor localizes to both the nucleus and cytoplasm, and upon ligand binding, translocates to the nucleus to initiate transcription.
• Post-translational modifications include phosphorylation, palmitoylation, and isopeptide bond formation; the protein contains zinc-finger DNA-binding domains essential for its function.
• The androgen receptor is mainly expressed in heart and skeletal muscle, with additional expression in basal and stromal cells of the prostate.
• Mutations in the AR gene are associated with androgen insensitivity syndrome (pseudohermaphroditism) and spinal bulbar muscular atrophy (neurodegeneration caused by triplet repeat expansion).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100), which is common to both full-length and known isoforms. Consider this when interpreting band patterns in western blot.
• For western blot, the predicted molecular weight is ~99 kDa. Use appropriate gel percentages and validate with positive controls such as LNCaP cell lysates.
• In immunohistochemistry or immunofluorescence, antigen retrieval protocols may be required; validate in relevant tissue types (e.g., prostate or muscle sections).
• Reported cross-reactivity with mouse and rat supports translational studies across species, but titration is recommended for each model.
• The antibody is suitable for multiple applications (WB, IP, IF-P, IHC-P, mIHC, ELISA); always include appropriate negative and positive controls in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-Androgen Receptor monoclonal antibody as part of a comprehensive portfolio of IVD raw materials for diagnostic and research laboratories. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare-target raw materials for androgen signaling and prostate cancer studies. Our one-stop supply chain helps streamline your transition from concept to clinic.