Anti-ANAPC7 Polyclonal Antibody for WB, ELISA - Q9UJX3

Core Product Specifications and Parameters

Biological Background: ANAPC7 Function and Localization

• ANAPC7 (APC7) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through the cell cycle. Related references: PMID:18485873, PMID:27120157, PMID:27509861
• APC/C mediates ubiquitination and subsequent degradation of target proteins, primarily forming Lys-11-linked polyubiquitin chains, and to a lesser extent Lys-48- and Lys-63-linked chains. Related references: PMID:18485873, PMID:29033132
• APC/C is activated by CDC20 in the metaphase/anaphase transition, targeting cyclin B and securin for degradation; it is inhibited by the mitotic checkpoint complex (MCC). Related references: PMID:27120157, PMID:27509861
• ANAPC7 exhibits enzyme-substrate adapter activity, mediating processive ubiquitination of specific substrates such as MKI67, which is important for brain development. Related references: PMID:34942119
• Subcellular localization includes the cytoplasm, cytoskeleton, nucleus, and spindle.
• The protein undergoes acetylation and alternative splicing, with a predicted molecular weight of approximately 63 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 50–150 of human ANAPC7; verify sequence homology if using non-human models, as cross-reactivity to mouse is expected.
• For Western blot, a band near 63 kDa is expected, but migration may shift due to post-translational modifications; validate with appropriate controls.
• Given ANAPC7's role in cell cycle regulation, consider using synchronized cell populations or cell cycle inhibitors to enhance detection.
• In ELISA, consider peptide competition assays to confirm specificity, as the immunogen region may influence binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies such as this anti-ANAPC7 antibody. We assist diagnostic manufacturers and researchers in the cell cycle and oncology fields with reliable sourcing and technical support. Contact us for bulk quantities, customized reagents, and ancillary raw materials to accelerate your assay development.