Anti-Amylin/DAP Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P10997

Core Product Specifications and Parameters

Biological Background: Amylin/DAP Function and Localization

• Islet amyloid polypeptide (IAPP), also known as amylin or diabetes-associated peptide (DAP), is encoded by the IAPP gene in humans.
• It functions as a glucoregulatory peptide hormone that contributes to energy homeostasis by selectively inhibiting insulin-stimulated glucose utilization and glycogen deposition in muscle, without affecting adipocyte metabolism. Related references: PMID:2690069
• IAPP signaling is mediated through amylin receptors (AMYRs), which are complexes of the calcitonin receptor (CALCR) and receptor activity-modifying proteins (RAMPs). It can also bind CALCR alone, though with lower affinity.
• The protein is secreted and is known for its propensity to form amyloid fibrils, which are implicated in type 2 diabetes pathology.
• Post-translational modifications include an intramolecular disulfide bond and C-terminal amidation, essential for biological activity.
• Keywords from UniProt: Amyloid, Hormone, Secreted, and structural features like 3D-structure and disulfide bond, underscoring its role in metabolic regulation and disease.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 34–70 of human amylin/DAP, corresponding to the mature peptide, suggesting the antibody may recognize both precursor and processed forms.
• For Western blot (WB), expect a band around 10 kDa; reducing conditions and protease inhibitors are recommended to prevent potential degradation.
• In immunohistochemistry (IHC-P), test the antibody on pancreatic islet sections, where amylin is co-secreted with insulin, and consider antigen retrieval protocols for optimal detection.
• For immunofluorescence (IF/ICC), validate the antibody in relevant cell lines that express IAPP, with appropriate fixation and permeabilization methods.
• In ELISA applications, pair this antibody with a suitable secondary antibody and optimize coating and detection conditions for quantitative amylin measurement.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting the entire development pipeline from concept to clinic. This anti-Amylin/DAP rabbit monoclonal antibody is ideal for metabolic disease research and diagnostic assay development. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your workflow.