Anti-AMY2A Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P04746

Core Product Specifications and Parameters

Biological Background: AMY2A Function and Localization

• AMY2A encodes pancreatic alpha-amylase, a secreted glycosidase also designated 1,4-alpha-D-glucan glucanohydrolase.
• The enzyme is secreted into the extracellular space, consistent with its digestive role in carbohydrate breakdown.
• AMY2A expression is detected specifically in the pancreas at the protein level.
• It belongs to the glycosyl hydrolase family 13, and its activity requires calcium and chloride ions, as indicated by metal-binding and ion-related keywords.
• Alternative splicing of the AMY2A gene produces multiple transcript variants, potentially giving rise to isoforms with distinct properties.
• Post-translational modifications include N-linked glycosylation and the formation of a pyrrolidone carboxylic acid residue at the N-terminus.
• The three-dimensional structure of AMY2A has been solved, providing insights into its catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 16–511 of human AMY2A, nearly the entire mature sequence. This broad coverage suggests the polyclonal antibody may recognize multiple linear and conformational epitopes.
• For Western blot (WB), the predicted molecular weight is ~58 kDa. Use freshly prepared pancreatic tissue lysate as a positive control, and consider reducing and non-reducing conditions to assess conformational epitopes.
• In immunofluorescence on paraffin-embedded sections (IF-P), optimize antigen retrieval conditions and validate staining specificity using appropriate tissue controls.
• The antibody cross-reacts with mouse and rat AMY2A orthologs according to the supplier; verify reactivity in your specific experimental model by comparing species-matched positive and negative controls.
• For ELISA, the polyclonal nature may provide high sensitivity; however, due to potential batch-to-batch variability, include an internal reference standard when comparing data across experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD raw materials, including validated polyclonal antibodies like this anti-AMY2A product for digestive enzyme and metabolic disorder research. Beyond antibodies, we offer a comprehensive portfolio of optimized monoclonals, bulk ancillary reagents, and custom antigen sourcing—bridging your project from concept through clinical assay development.