Anti-AMY1A Rabbit Polyclonal Antibody for WB, ELISA - P04745

Core Product Specifications and Parameters

Biological Background: AMY1A Function and Localization

• The target protein is encoded by the human AMY1A gene (GeneID: 276), which belongs to the alpha-amylase family and is involved in carbohydrate digestion.
• The immunogen corresponds to amino acids 16-200 of the canonical isoform (NP_004029.2), representing the N-terminal region of the enzyme.
• AMY1A is highly expressed in salivary glands and is responsible for the initial breakdown of starch into sugars in the oral cavity.
• The UniProt entry P04745 is currently annotated as inactive, limiting the availability of detailed curated functional annotations.
• Based on sequence conservation, the antibody is expected to cross-react with mouse and rat orthologs, supporting multispecies studies.
• Alpha-amylase activity is critical for metabolic homeostasis, and AMY1A copy number variations have been associated with dietary adaptations and obesity risk.

Experimental Guidance and Technical Tips

• The immunogen was designed as a recombinant fusion protein spanning the N-terminal domain (aa 16-200), which is likely to present linear epitopes suitable for denaturing WB conditions.
• For WB, validate detection of a band near 57 kDa using positive control lysates from human salivary glands or recombinant AMY1A protein. Include mouse and rat tissue lysates to confirm cross-reactivity.
• For ELISA, consider testing both direct and sandwich formats; optimize coating with recombinant AMY1A protein or native amylase from saliva.
• Due to the polyclonal nature, lot-to-lot consistency should be verified for critical quantitative applications.
• Pre-adsorption against the immunogen or relevant species serum may be considered to reduce background in sensitive assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers, research laboratories, and IVD kit manufacturers with streamlined access to critical raw materials. For projects targeting amylase enzymes and carbohydrate metabolism, we can supply validated antibody pairs, bulk ancillary reagents, and customized purification services. Our network enables efficient sourcing of rare-target reagents and seamless scale-up, accelerating your path from concept to clinical application.